ZetaSizer Ultra - Malvern
The ZetaSizer Ultra from the Malvern company provides the ability to measure the size and zeta potential of particles, molecules or micelles in a liquid medium. The unique features of the ZetaSizer for measurements over a wide concentration range and precise temperature control give reproducible, repeatable and accurate results. This device utilizes Multi-Angle Dynamic Light Scattering (MADLS®) technology for the highest resolution of sizing data. The ZetaSizer works in the sample temperature range of 0–120 °C. The minimum sample volume is 3 μL for the particle size and 20 μL for the zeta potential measurement. The minimum sample concentration for particle size measurement depends on the technique used, starting from 0.1 mg/mL for Dynamic light scattering (Non-Invasive Back-Scatter) to 1 mg/mL for MALDS, the same concentration as for zeta potential. The size range is 0.3–10 μm for size determination and 3.8 nm–100 um for zeta potential measurement.
Specification
Specification
A) Particle size and molecular size
| Measurement principle |
Non-Invasive Back Scatter (NIBS) Dynamic Light Scattering |
|---|---|
| Measurement angle | 173°, 13°, 90° |
| Measurement range | Diameter: 0.3 nm – 15 μm (2) (9) |
| Minimum sample volume | 3 μL |
| Concentration range |
Minimum sample concentration: Blue Label: 0.2 mg/mL 15 kDa Protein Red Label: 0.1 mg/mL 15 kDa Protein MADLS: 0.1 mg/ml 15 kDa Protein MADLS Particle Concentration Enabled? Blue Label: No Red Label: Yes Maximum sample concentration (3): 40% w/v |
B) Zeta potential
| Measurement principle |
Mixed-Mode Measurement phase analysis light scattering (M3-PALS) |
|---|---|
| Size range | 3.8 nm – 100 μm |
| Minimum sample volume | 20 μL (4) |
| Concentration range |
Red Label: 1 mg/mL (5) to 40% w/v (6) Blue Label: 10 mg/mL (5) to 40% w/v (6) |
| Sample conductivity range |
Maximum: 260 mS/cm |
C) Static Light Scattering (molecular weight)
| Molecular weight range |
300 Da – 20 mDa |
|---|---|
| Measurement principle | Static Light Scattering with Debye Plot |
| Accuracy | +/- 10% typical |
D) Particle concentration
| Measurement principle |
Blue Label: N/A Red Label: MADLS - Particle Concentration (Mie) |
|---|---|
| Concentration range |
Blue Label: N/A Red Label: 1x108 to 1x1012 particles/mL |
| Calibration type |
Blue Label: N/A Red Label: Single point system calibration only |
E) System
| Detector |
Avalanche Photodiode |
|---|---|
| Laser safety |
He-Ne (633 nm) |
| Temperature control range | 0°C to 120°C (7) |
| Ambient conditions |
+10°C to +35°C, 35 - 80% RH (non-condensing) |
F) Notes
| Notes |
(1) Water as dispersant |
|---|
Publications
POLÁKOVÁ, V.; MATULOVÁ, J.; BRTNÍKOVÁ, J.; FOHLEROVÁ, Z.; ŠMERKOVÁ, K.; KAISER, J.; ZIKMUND, T.; PROCHÁZKOVÁ, P.; ŽÍDEK, J.; VOJTOVÁ, L., 2025: In Situ Homogeneous Generation of Copper Nanoparticles in Collagen-Cellulose Freeze-Dried Foams Using Natural Reduction Agents to Enhance Their Stability, Antibacterial Properties, and Cytocompatibility. ACS OMEGA 10 (35), p. 39799 - 39813, doi: 10.1021/acsomega.5c03661 (MIRA-STAN, ZETASIZER, LEICACOAT-STAN, micro-CT-L240)
Hashemi, A.; Ezati, M.; Zumberg, I.; Chmelíková, L.; Fohlerová, Z.; Provazník, V., 2025: In vitro evaluation of 3D-printed conductive chitosan – polyaniline scaffolds with exosome release for enhanced angiogenesis and cardiomyocyte protection. RSC ADVANCES 15, p. 16826 - 16844, doi: 10.1039/d5ra02940f (TITAN, FTIR, ZETASIZER)
TINOCO NAVARRO, L.; CIHLÁŘ, J.; MICHALIČKA, J.; KAŠTYL, J.; ČÁSTKOVÁ, K., 2023: Effect of MCAA Synthesis and Calcination Temperature on Heterojunction Formation and Photocatalytic Activity of Biphasic TiO2 (B/A). CATALYSIS LETTERS 2, doi: 10.1007/s10562-023-04489-7 (RIGAKU3, WITEC-RAMAN, FTIR, VERIOS, TITAN, KRATOS-XPS, BET-DEGASSER, BET-ANAMET, ZETASIZER)
KADLECOVÁ, Z.; SEVRIUGINA, V.; LYSÁKOVÁ, K.; RYCHETSKÝ, M.; CHAMRADOVÁ, I.; VOJTOVÁ, L., 2023: Liposomes Affect Protein Release and Stability of ITA-Modified PLGA-PEG-PLGA Hydrogel Carriers for Controlled Drug Delivery. BIOMACROMOLECULES 25 (1), p. 67 - 10, doi: 10.1021/acs.biomac.3c00736 (DAWN-HELEOS, ZETASIZER, NMR, MIRA-STAN)
VISHAKHA, V.; ABDELLATIF, A.; MICHALIČKA, J.; WHITE, P.; LEPCIO, P.; TINOCO NAVARRO, L.; JANČÁŘ, J., 2023: Carboxymethyl starch as a reducing and capping agent in the hydrothermal synthesis of selenium nanostructures for use with three-dimensional-printed hydrogel carriers. ROYAL SOCIETY OPEN SCIENCE 10 (10), p. 1 - 17, doi: 10.1098/rsos.230829 (ZETASIZER, RIGAKU3, VERIOS, MIRA-STAN, LEICACOAT-STAN, TITAN, FTIR-CHEMLAB, KRATOS-XPS, JASCO)